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Saturday, July 18, 2020 | History

2 edition of isolation of bacteriophage from sewage and their characterization by electron microscopy. found in the catalog.

isolation of bacteriophage from sewage and their characterization by electron microscopy.

Alan D. Darlington

isolation of bacteriophage from sewage and their characterization by electron microscopy.

by Alan D. Darlington

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Published .
Written in English


Edition Notes

ContributionsManchester Polytechnic. Department of Biological Sciences.
ID Numbers
Open LibraryOL16672598M

Isolation of bacteriophage Bacteriophages were isolated from raw sewage samples obtained from Al-Sadar hospital raw sewage in Basra according to previously described methods [20,21]. A ml volume of fresh sewage was mixed with 20 ml of bacteriophage broth [peptone ( g/L), beef extract. Isolation and Characterization of Bacteriophages from of the filtered lysate prior to further characterization. Bacteriophages from the inoculated and uninoculated tanks are shown in Table 1. Phages active against the LA10 starter To generate high-titer phage lysates for electron microscopy.

Phage isolation, morphology and storage stability One lytic phage against P. aeruginosa strain Lfar01 was isolated from sewage and a homogeneous stock prepared after three rounds of purification. We named this virus Pseudomonas phage BrSP1. Electron microscopy of phage BrSP1 showed the long tail structure typical of. Isolation of Novel Large and Aggregating Bacteriophages Philip Serwer, Shirley J. Hayes, Julie Thomas, Borries Demeler, and Stephen C. Hardies Section 2. Bacteriophage Characterization 7. Enumeration of Bacteriophages by Double Agar Overlay Plaque Assay Andrew M. Kropinski, Amanda Mazzocco, Tom Waddell, Erika Lingohr, and Roger P. Johnson : $

  A sampling of topics from the first volume: bacteriophage enrichment from water and soil, isolation of phage via induction of lysogens, enumeration of bacteriophages by double agar overlay plaque assay, basic phage electron microscopy, construction of phage mutants, generalized transduction, and preparation and characterization of anti-phage serum.   Bacteriophage characterization. Phage morphology and virion size were characterized by Transmission Electron Microscopy using a JEOL JSM CXII electron microscope. Grids were negatively stained with uranyl acetate (2% w/v). Images were .


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Isolation of bacteriophage from sewage and their characterization by electron microscopy by Alan D. Darlington Download PDF EPUB FB2

BACTERIOPHAGE FROM RAW SEWAGE SPECIFIC FOR Escherichia coli OH7 Ms. Faizah and Mr. Masrul for their assistance in electron microscopy. Besides, I would like to thank the Institute of ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGE FROM RAW. Isolation and characterization of bacterial host strains.

Sediment sample plus the overlying water were collected (March, ) into sterile jars, capped on site and preserved in cooled boxes for transportation to the molecular laboratory in Jomo Kenyatta University of Agriculture and Technology (JKUAT).Cited by: 1.

Further purification and characterization are achieved by density gradients, electron microscopy studies, and genomic analysis. This straightforward methodology can be applied to the detection, enumeration, and isolation of bacteriophages from any bacterial species, using the appropriate host strain, media, and culture conditions.

PMID: Author: Juan Jofre, Maite Muniesa. The vB-EcoS bacteriophage was isolated as a virus infecting E. coli from samples of urban sewage. The isolation procedure was based on the one-host enrichment method in which a raw urban sewage sample was mixed with a culture of E. coli MG strain to obtain the lysate of vB-EcoS bacteriophage, as described in the Material and Methods Cited by: 4.

This study describes the isolation and characterization of three Nocardia bacteriophages NOC1, NOC2, and NOC3 for the control of Nocardia species. The bacteriophages isolated in this study have shown promising results in controlling foam producing bacterial growth under laboratory conditions, suggesting that it may prove useful in the field as Cited by: 7.

Isolation and characterization of Salmonella bacteriophages from poulty and pig sewage wastes Article (PDF Available) in The Indian journal of animal sciences 87(5).

Bacteriophage Isolation. Front Matter. Pages PDF. Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and Bacteria Microbial ecology Microbial evolution Morphology Pathogenicity Termination electron microscopy molecular biology.

Editors and affiliations. Two phages of Escherichia coli OH7 from a sewage sample were isolated and characterized to control E.

coli OH7 in foods. The isolated phages, BECP2 and BECP6, belonged to the Myoviridae and Podoviridae, respectively. Their burst sizes were 33 PFU/cell for BECP2 and 51 PFU/cell for BECP6 after a latent period of 25 min.

Shiga toxin genes were not detected on either the. Host ranges and morphological characterization of phages. Once suitable S. aureus hosts had been isolated, the next step was to screen bacteriophages and assess their lytic abilities and host ranges.

Sewage influent was chosen as the phage source because it has yielded a wide diversity of phages in the past. Fifty-two staphylococcal phages. Isolation and spot test of bacteriophages. Eighty-seven samples, including catheter washings, wastewater from drainages and raw sewage collected from a local hospital were centrifuged ( × g, 10 min at 4 °C) and the supernatants were filtered through a μm-pore-size membrane.

Each of the filtered supernatants was examined for the. Abstract. Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins.

Adsorption rate as well as single step growth curve experiments showed variation among phages. Isolation from Sewage Influent and Characterization of Novel Transmission electron microscopy involves bacteriophages (phages) and their derivatives, and a number of promising candidates have been described (2, 5, 7, 13, 17, 18, 27), notably bacteriophage K.

Isolation and Characterization of Bacteriophage from Waste W ater Against E. coli, a Food Borne observed within 4- 6 hours against host bacteria and are found to be lytic phage with Plaque. isolation, purification, and molecular identify- cation of a nitrogen-fixing bacterium occurred in some rhizosphere soil of Taif region.

The presence of bacteriophage(s) specific to the nitrogen-fixing bacterial strain(s) and their electron microscopy was also aimed. PCR isolation. All isolated bacteriophages exhibited a high specificity, being able to lyse only C. jejuni, but not other Gram–negative bacteria, ,Escherichiacoli,Salmonellaspp.,andGram–iophagescontain an icosahedral head and a contractile tail sheath in transmission electron microscopy, and possess ds.

Therefore, there is an urgent need to develop novel antibacterial agents to control this pathogen. Bacteriophages are a potential alternative treatment for MRSA infections.

Objectives: The objective of this study was the isolation and characterization of MRSA phages from the sewage at a tertiary pediatric hospital.

//ce:title Isolation and characterization of a bacteriophage and its utilization against multi-drug resistant Pseudomonas aeruginosa //dc:description Abstract Aims To identify, isolate, and characterize a lytic bacteriophage against the multiple-drug resistant clinical strain of Pseudomonas aeruginosa and to determine the phage efficacy against the bacterial.

Isolation of bacteriophages: The enrichment method of Cerveny et al. [17] was adopted for the isolation of phages specific to P. aeruginosa PAO from sewage samples. Samples were collected either from drainage of different localities in the town or from fresh water lakes around Chandigarh. These sites were selected to isolate.

the aim of this study was to isolate bacteriophage from sewage water of udsm oxidation ponds capable of lysing enteric And plaques was the only way to determine if the project has been successfull. Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community.

Bacteriophages: Methods and Protocols pulls together the vast body of. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined.

Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family.Staphylococcus aureus is a Gram-positive bacterium that causes a variety of diseases, including bovine mastitis, which has severe economic consequences.

Standard antibiotic treatment results in selection of resistant strains, leading to need for an alternative treatment such as bacteriophage therapy. Present study describes isolation and characterization of a staphylococcal phage from sewage.

How to cite this article: Li, E. et al. Isolation and characterization of a bacteriophage phiEap-2 infecting multidrug resistant Enterobacter aerogenes. Sci. Rep. 6, ; doi: /srep